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  1. . , 30 , , .
  2. Muirhead H., Perutz M. Structure of hemoglobin. A three-dimensional Fourier synthesis of reduced human hemoglobin at 5.5 A resolution // Nature : . 1963. . 199. № 4894. . 633638.
  3. Kendrew J., Bodo G., Dintzis H., Parrish R., Wyckoff H., Phillips D. A three-dimensional model of the myoglobin molecule obtained by x-ray analysis // Nature : . 1958. . 181. № 4610. . 662666.
  4. Leicester, Henry. «Berzelius, Jöns Jacob». Dictionary of Scientific Biography 2. New York: Charles Scribners Sons. 9097 (1980). ISBN 0-684-10114-9
  5. . . . .  , 1987.
  6. // .  , 1988.
  7. 1 2 N. H. Barton, D. E. G. Briggs, J. A. Eisen. «Evolution», Cold Spring Harbor Laboratory Press, 2007  P. 38. ISBN 978-0-87969-684-9
  8. Fulton A, Isaacs W. (1991). «Titin, a huge, elastic sarcomeric protein with a probable role in morphogenesis». Bioessays 13 (4): 157161. PMID 1859393.
  9. EC 3.4.23.1  pepsin A
  10. S J Singer. The Structure and Insertion of Integral Proteins in Membranes. Annual Review of Cell Biology. Volume 6, Page 247296. 1990
  11. . 3 .  .: , 1984
  12.   .
  13. 1 2 . . .  ., 1987.  544 .
  14. . , 3 .  .: , 1985.
  15. 1 2 2. (« », , 1996 1997)
  16. http://pdbdev.sdsc.edu:48346/pdb/molecules/pdb50_6.html
  17. Anfinsen C. (1973). «Principles that Govern the Folding of Protein Chains». Science 181: 223229. . , , « , [] [] ».
  18. Ellis RJ, van der Vies SM. (1991). «Molecular chaperones». Annu. Rev. Biochem. 60: 321347. DOI:10.1146/annurev.bi.60.070191.001541. PMID 1679318.
  19. Sun Y, MacRae TH. (2005). «The small heat shock proteins and their role in human disease». FEBS J. 60: 26132627. PMID 115943797.
  20. Wilken J, Kent SB. Chemical protein synthesis. Curr Opin Biotechnol. 1998.9(4):412426
  21. Dawson PE, Kent SB. Synthesis of native proteins by chemical ligation. Annu Rev Biochem. 2000;69:923960
  22. Dobson CM. (2000). The nature and significance of protein folding. In Mechanisms of Protein Folding 2nd ed. Ed. RH Pain. Frontiers in Molecular Biology series. Oxford University Press: New York, NY.
  23. Stack D, Neville C, Doyle S. Nonribosomal peptide synthesis in Aspergillus fumigatus and other fungi. Microbiology. 2007 May; 153(Pt 5):12971306
  24. Welker M, von Döhren H. Cyanobacterial peptides  natures own combinatorial biosynthesis. FEMS Microbiol Rev. 2006 Jul; 30(4):530563
  25. Demartino GN, Gillette TG. Proteasomes: machines for all reasons. Cell. 2007 May 18. 129(4):659662
  26. Bronner C, Chataigneau T, Schini-Kerth VB, Landry Y. The «Epigenetic Code Replication Machinery», ECREM: a promising drugable target of the epigenetic cell memory. Curr Med Chem. 2007;14(25):26292641
  27. Yannay-Cohen N, Razin E. (2000). «Translation and transcription: the dual functionality of LysRS in mast cells». Mol Cells. 22: 127132. PMID 17085962.
  28. Bairoch A. (2000). «The ENZYME database in 2000». Nucleic Acids Res 28: 304305. PMID 10592255.
  29. Radzicka A, Wolfenden R. (1995). «A proficient enzyme». Science 6 (267): 9093. PMID 7809611.
  30. The Catalytic Site Atlas at The European Bioinformatics Institute
  31. Erickson HP. Evolution of the cytoskeleton. Bioessays. 2007:668677
  32. Wolberg AS (2007). «Thrombin generation and fibrin clot structure». Blood Rev. 21(3): 131142. PMID 17208341.
  33. . , .-. . . .: , 2000, . 308309.
  34. J. Li, D. R. Barreda, Y.-A. Zhang, H. Boshra, A. E. Gelman, S. LaPatra, L. Tort & J. O. Sunyer (2006). «B lymphocytes from early vertebrates have potent phagocytic and microbicidal abilities». Nature Immunology 7: 11161124. PMID 16980980.
  35. Felix NJ, Allen PM. Specificity of T-cell alloreactivity. Rev Immunol. 2007 Dec; 7(12):942953
  36. Hinnebusch AG. Translational regulation of GCN4 and the general amino acid control of yeast. Annu Rev Microbiol. 2005;59:407450
  37. Anderson P, Kedersha N. RNA granules. Cell Biol. 2006:172(6):803808
  38. . ., . ., . .   . . 2007  38(3):4046
  39. Wittenberg JB. On optima: the case of myoglobin-facilitated oxygen diffusion. Gene. 2007 Aug 15. 398(12):156161.
  40. Driessen AJ, Nouwen N. Protein Translocation Across the Bacterial Cytoplasmic Membrane. Annu Rev Biochem. 2007 Dec 13 [Epub ahead of print]
  41. Drory O, Nelson N. (2006). «The emerging structure of vacuolar ATPases». Physiology (Bethesda). 21: 317325. PMID 16990452.
  42. Dupré DJ, Hébert TE. Biosynthesis and trafficking of seven transmembrane receptor signalling complexes. Cell Signal. 2006;18(10):15491559
  43. Karp G. Cell and Molecular Biology: Concepts and Experiments, Fourth ed, pp. 346358. John Wiley and Sons, Hoboken, NJ. 2005.
  44. Schroer, Trina A. Dynactin. Annual Review of Cell and Developmental Biology. 2004 20, 759779. PMID 15473859
  45. Voet D, Voet JG. Biochemistry Vol 1 3rd ed., Hoboken, NJ (2004).
  46. Brosnan J. (2003). «Interorgan amino acid transport and its regulation». J Nutr 133 (6 Suppl 1): 2068S-72S. PMID 12771367.
  47. . ? / . . .. , .: , 2009, 176 ., ., ISBN 978-5-9650-0057-9
  48. .. . ., , 1988
  49. Huber R. Nature 280 565 (1979)
  50. Richards FM Annu. Rev. Biophys. Bioeng. 6 151 (1977)
  51. .. 32 742 (1987)
  52. .., .. . 17 577 (1983)
  53. .., .., .. , 17 543 (1983)
  54. Doster W, Cusack S, Petry WNature 337 754 (1989)
  55. Parak F, Frolov E N, Moessbauer R L, Goldanskii V I J. Mol. Biol. 145 825 (1981)
  56. .., .. , 30 517, (1985)
  57. Parak F, Heidemeier J, Knapp W, in Biological and Artificial Intelligence Systems (Eds E Clementi, S Chin) (Leiden: ESCOM, 1988) p. 23
  58. .. . 32 761, (1987)
  59. Krupyanskii Yu F et al. Z. Naturforsch. C 37 57 (1982)
  60. Goldanskii V I, Krupyanskii Y F, Fleurov V N Phys. Scripta 33 527 (1986)
  61. Goldanskii V I, Krupyanskii Yu F Quart. Rev. Biophys. 22 39 (1989)
  62. Krupyanskii Yu F et al. Hyperfine Interact. 53 59 (1990)

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  • ., ., . . . 3 .  .: , 1994.  ISBN 5-03-001986-3
  • . . 3 .  .: , 1985.
  • . . 3 .  .: , 1984.

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